The Tien
Group
Boston University
Department of Biomedical Engineering
44 Cummington Mall
Boston, MA 02215
We are an interdisciplinary group of
researchers who invent new types of biomaterials, for uses that range from
basic studies of quantitative physiology to clinical applications in
regenerative medicine. Of particular
interest are perfusable, microfluidic materials whose internal geometries mimic
the organization of native vascular networks.
With these materials, we seek to solve one of the long-standing
challenges in tissue engineering: how to form clinically relevant volumes of
tissue that are nourished and drained by functional microvessels. We are also interested in developing
vascularized microphysiological systems for studying vessel-tissue interactions
in vitro, particularly in the context of cancer progression. We are currently in our 22nd year
at Boston University.
Our research addresses the following
questions:
·
How
does one synthesize and vascularize perfusable
biomaterials?
·
What
principles govern vascularization of biomaterials? Can one distill these principles into a
computer algorithm for rational scaffold design?
·
How
does one scale up vascularized materials to clinically relevant sizes? How do such materials behave upon perfusion
in vivo?
To answer these questions, we develop
unconventional methods to organize vascular and non-vascular cells and
extracellular components into perfused, micropatterned tissues. We use traditional techniques of microvascular
physiology (along with a healthy mixture of ideas from vascular cell biology,
transport phenomena, biomechanics, and numerical modeling) to analyze, predict,
and control the behavior of these tissues.
Below, we invite you to read about the
group and its research interests, publications, and resources. For further information, please contact us
directly.
GROUP INFORMATION
Principal
Investigator: Joe Tien
Address: We are located on the
7th floor of the Engineering Research Building (44 Cummington
Mall), in rooms 713, 715, and 717. [Map]
Phones: (617) 358-3055 [Joe’s
office—ERB 717 & ERB 307]
(617) 358-2831 [Main
lab—ERB 715]
(617) 353-5557 [Lounge—ERB
713]
Fax: (617) 358-2835
RESEARCH INTERESTS
Mechanics
of vascularization
Much
of our work is focused on understanding how vascularization of microfluidic
scaffolds occurs and what factors control its long-term success. We have found that the microfluidic structure
of a scaffold is important in controlling the initial formation of open
vessels, but it is insufficient to guarantee sustained perfusion. Through a combination of experimental and
computational studies, we have shown that mechanical stresses at the
cell-scaffold interface determine whether the vascular lining remains adherent
or detaches over time. We have begun to
apply this physical theory of vascularization to a variety of challenging
problems, including the vascularization of capillary-scale channels and the
formation of functional lymphatic microvessels in which the mechanical stresses
are inherently destabilizing.
Techniques for patterning biological
materials
We
have a long-standing interest in inventing new techniques for patterning
biological materials. In fact, our work
on vascularization is based on subtractive methods that we developed in-house
to create single channels and entire networks within hydrogels of extracellular
matrix. We are always interested in
patterning scaffolds with ever finer resolution, greater three-dimensional
connectivity, and larger network sizes.
Current efforts are focused on specific applications, including the
development of valves that can actively pump fluids and the creation of large-scale
microfluidic scaffolds suitable for composite tissue engineering.
Engineering
vascularized microphysiological systems
Our
latest work is geared towards developing microphysiological systems
("organs-on-a-chip") that model the interactions of vessels and
non-vascular tissue in human disease. We
have created microfluidic models of human breast cancer and its progression
towards vascular invasion, an obligate step in metastasis. These models provide a well-controlled system
for testing whether a particular element of the tumor microenvironment, such as
interstitial flow or the presence of adipose cells, alters the rate at which
tumor cells escape into a nearby vessel.
We have also begun to develop vascularized models of human obesity,
which can be used to understand the interplay between breast cancer, obesity,
and vascular invasion.
MEMBERS (Current in bold)
|
Joe Tien |
jtien | bu_edu |
Principal
investigator |
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|
Owen Kelly |
okelly | bu_edu |
Undergraduate researcher |
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|
Nikhil Lahiri |
lahirini | bu_edu |
Undergraduate researcher |
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|
Alex Seibel |
aseibel | bu_edu |
Doctoral student |
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|
Abed Tlemcani |
arhmari | bu_edu |
Undergraduate researcher |
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Chitrangada
Acharya (2010P) |
|
Research scientist,
Allied Innovative Systems |
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|
Wajd
Al-Holou (2002U) |
|
M.D.,
University of Michigan, with specialization in neurosurgery (2009) |
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|
Nelson Boland (2013-2015U) |
M.D., Baylor College of
Medicine (2019) |
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|
(2011-2012M) |
M.S. thesis: "Genipin Crosslinked Collagen
Microfluidic Scaffolds Form Stable Microvessels In Vitro Using Human
Endothelial Cells" M.D., Albert Einstein
College of Medicine (2016) |
|
||||
|
Kenneth
Chrobak (2003-2007D) |
Ph.D.
thesis: "Formation of Perfused
Microvessels In Vitro, and Their Use as Models of Barrier Function" Research manager, Baxter
Healthcare |
|
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|
Cassandra Chua (2017-2020U) |
Medical student, Boston
University |
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(2012U) |
Ph.D., biomedical
engineering, Cornell University (2018), with Larry Bonassar Engineer, 3DBio
Therapeutics |
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|
Brent Coisman (2013-2014U) |
Research scientist,
Bluebird Bio |
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|
Russell
Condie (2006U) |
|
Doctoral
student in biomedical engineering, University of Utah |
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|
(2014-2015U) |
Ph.D., biomedical
engineering, Case Western Reserve University (2020), with Stathis
Karathanasis Postdoctoral fellow with
Paula Hammond (MIT) |
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|
Yoseph
Dance (2018-2022D) |
Ph.D.
thesis: " Engineering 3D Breast
Tumor-on-a-Chips to Investigate the Roles of Adipose Tissue and Obesity in
the Early Stages of Breast Cancer Metastasis" |
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|
Hillary
Eggert (2004U) |
|
Carthage
College, Biology |
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Evan Feldman (2014-2015U) |
Software engineer,
Viridis3D |
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|
Andrew
Golden (2002-2008D) |
Ph.D.
thesis: "Microfluidic Hydrogels
for Microvascular Tissue Engineering" Research manager, AgaMatrix |
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|
John
Jiang (2017-2019S) |
Animal
surgeon, Boas Lab, Boston University |
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|
(2011-2013U) |
Doctoral student in
biophysics, UC Santa Barbara |
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Alex Leung
(2009-2011U) |
|
M.D., Boston University,
with specialization in vascular surgery (2016) |
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|
Henry
Li (2015-2019D) |
Ph.D.
thesis: "Engineering Components of
a Vascularized, Microsurgically Implantable Adipose
Tissue" Research
manager, WuXi Biologics |
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|
(2014-2016U) |
Ph.D., biomedical engineering,
Johns Hopkins University (2021), with Peter Searson Postdoctoral fellow with
Myriam Heiman (Broad
Institute) |
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|
Chao Liu (2017-2019U) |
Doctoral
student in biomedical engineering, Case Western Reserve University |
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|
(2014-2018U) |
Doctoral student in
biomedical engineering, University of Michigan |
|||||
|
Jordann
Marinelli (2015-2017U) |
Consultant, Accenture |
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Brandon Markway (2002U) |
Ph.D.,
biomedical engineering, Oregon Health and Science University (2010), with
Owen McCarty and Monica Hinds Research scientist, Aronora |
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|
Miles Massidda (2017-2018U) |
Doctoral student in
biomedical engineering, UT Austin |
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|
Cate
McCullough (2003U) |
|
M.S.,
bioengineering, Stanford University (2007) Manager, Durango
Machining Innovations |
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|
(2015-2018U) |
Doctoral student in
biomedical engineering, Case Western Reserve University |
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|
Mackenzie
Obenreder (2020-2022U) |
Doctoral
student in chemical and biological engineering, CU Boulder |
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|
Neil
Parikh (2018-2020U) |
|
Medical
student, Boston University |
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|
Gavrielle
Price (2004-2009D) |
|
Ph.D.
thesis: "Mechanical and Chemical
Control of Barrier in Engineered Microvessels" Science writer, Ironwood
Pharmaceuticals |
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Jason Pui (2011-2012U) |
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Engineer, Accellent |
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Rachel Roesch (2011U) |
|
Ph.D.,
chemistry, University of Pennsylvania (2017), with Feng
Gai Research
scientist, Illumina |
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|
(2014-2017U) |
|
Medical
student, Boston University School of Medicine |
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(2008U) |
Ph.D., biomedical
engineering, UT Austin (2016), with Nicholas Peppas Scientist, Dow Corning |
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|
(2002-2005D) |
|
Ph.D.
thesis: "In Vitro Engineering of a
Microvascular Network" Assistant professor of
pediatrics, University of Connecticut Health Center |
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|
Rebecca Thompson (2012-2015U) |
|
Research scientist,
Broad Institute |
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|
James Truslow (2006-2008M; 2008-2011D;
2011-2013P) |
Ph.D. thesis: "Design and Analysis of Engineered
Microvasculature via Computational Methods" M.S. thesis: "Drainage Systems That Maintain
Transmural Pressure in Engineered Microvascular Tissue" Research scientist,
Brigham & Women's Hospital |
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|
Kim
Waller (2007-2008U) |
|
Ph.D.,
biomedical engineering, Brown University (2013), with Gregory Jay Program manager, Ximedica |
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|
(2007-2012D; 2012P) |
Ph.D. thesis: "Normalization of Microvascular
Physiology in Engineered Microvessels via Cyclic Adenosine Monophosphate
Supplementation and Artificial Lymphatic Drainage" Research scientist,
Catamaran Bio |
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|
Jingyi
Xia (2017-2019U) |
Doctoral student in
biomedical engineering, University of Michigan |
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|
Jing Xu (2014-2016U; 2016-2018S) |
Medical student,
University of Massachusetts |
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(P: postdoctoral fellow; D:
doctoral student; M: Master's student; U: undergraduate
researcher; S: research staff)
PUBLICATIONS
69. Tien, J.
& Dance, Y.W. Protein-based microfluidic models for biomedical
applications. In Handbook of the
Extracellular Matrix: Biologically-Derived Materials (eds. Maia, F.R.,
Reis, R.L. & Oliveira, J.M.), in press (Springer, Berlin).
68. Dance, Y.W., Obenreder, M.C., Seibel,
A.J., Meshulam, T., Ogony, J.W., Lahiri,
N., Pacheco-Spann, L., Radisky, D.C., Layne, M.D.,
Farmer, S.R., Nelson, C.M. & Tien, J., Adipose
cells induce escape from an engineered human breast microtumor
independently of their obesity status. Cell. Mol. Bioeng. 16, 23-39 (2023). [PDF
+ Supporting Information]
67. Seibel, A.J., Kelly, O.M., Dance, Y.W.,
Nelson, C.M. & Tien, J., Role of lymphatic
endothelium in vascular escape of engineered human breast microtumors.
Cell. Mol. Bioeng. 15, 553-569 (2022).
[PDF + Supporting Information]
66. Dance, Y.W., Meshulam,
T., Seibel, A.J., Obenreder, M.C., Layne, M.D.,
Nelson, C.M. & Tien, J., Adipose stroma
accelerates the invasion and escape of human breast cancer cells from an
engineered microtumor. Cell. Mol. Bioeng. 15, 15-29 (2022). [PDF + Supporting Information]
65. Tien, J.
& Ghani, U. Methods for forming human lymphatic
microvessels in vitro and assessing their drainage function. In
Biomedical Engineering Technologies,
Volume 2 (Methods in Molecular Biology, vol. 2394) (eds. Rasooly, A., Baker, H. & Ossandon,
M.R.), pp. 651-668 (Humana Press, Totowa, NJ, 2022). [PDF]
64. Tien, J.,
Dance, Y.W., Ghani, U., Seibel, A.J. & Nelson,
C.M., Interstitial hypertension suppresses escape of human breast tumor cells
via convection of interstitial fluid. Cell. Mol. Bioeng. 14, 147-159 (2021). [PDF
+ Supporting Information]
63. Tien, J.
& Dance, Y.W., Microfluidic biomaterials. Adv. Healthcare Mater. 10, 2001028 (2021).
[PDF]
62. Rabie, E.M.,
Zhang, S.X., Kourouklis, A.P., Kilinc,
A.N., Simi, A.K., Radisky, D.C., Tien,
J. & Nelson, C.M., Matrix degradation and cell proliferation are coupled to
promote invasion and escape from an engineered human breast microtumor.
Integr. Biol. 13, 17-29
(2021). [PDF +
Supporting Information]
61. Tien, J., Li,
X., Linville, R.M. & Feldman, E.J., Comparison of blind deconvolution- and Patlak analysis-based methods for determining vascular
permeability. Microvasc. Res. 133,
104102 (2021). [PDF] [Supporting
Information]
60. Tien, J., Ghani, U., Dance, Y.W., Seibel, A.J., Karakan,
M.C., Ekinci, K.L. & Nelson, C.M., Matrix pore
size governs escape of human breast cancer cells from a microtumor
to an empty cavity. iScience 23, 101673 (2020). [PDF + Supporting Information]
59. Li, X., Xu,
J., Bartolák-Suki, E., Jiang, J. & Tien, J., Evaluation of
1-mm-diameter endothelialized dense collagen tubes in vascular microsurgery. J.
Biomed. Mater. Res. B 108, 2441-2449 (2020). [PDF]
58. Tien, J.,
Tissue engineering of the microvasculature. Compr. Physiol. 9,
1155-1212 (2019). [PDF]
57. Li, X., Xia, J., Nicolescu, C.T., Massidda, M.W., Ryan, T.J. & Tien,
J., Engineering of microscale vascularized fat that
responds to perfusion with lipoactive hormones. Biofabrication 11, 014101 (2019). [PDF]
56.
Thompson, R.L., Margolis, E.A.,
Ryan, T.J., Coisman, B.J., Price, G.M., Wong, K.H.K.
& Tien, J., Design principles for lymphatic
drainage of fluid and solutes from collagen scaffolds. J. Biomed. Mater. Res. A 106,
106-114 (2018). [PDF]
55. Li, X., Xu, J., Nicolescu, C.T., Marinelli, J.T.
& Tien, J., Generation, endothelialization, and
microsurgical suture anastomosis of strong 1-mm-diameter collagen tubes. Tissue Eng. A 23, 335-344 (2017). [PDF]
54. Piotrowski-Daspit, A.S., Simi, A.K., Pang, M.-F., Tien, J. & Nelson, C.M. A
three-dimensional culture model to study how fluid pressure and flow affect the
behavior of aggregates of epithelial cells. In Mammary
Gland Development (Methods in Molecular Biology, vol. 1501) (eds. Martin,
F., Stein, T. & Howlin, J.), pp. 245-257 (Humana
Press, New York, NY, 2017). [PDF]
53. Piotrowski-Daspit,
A.S., Tien, J. & Nelson, C.M., Interstitial fluid
pressure regulates collective invasion in engineered human breast tumors via Snail, vimentin,
and E-cadherin. Integr. Biol. 8, 319-331 (2016). [PDF + Supporting Information]
52. Linville, R.M., Boland, N.F., Covarrubias, G., Price, G.M.
& Tien, J., Physical and chemical signals that
promote vascularization of capillary-scale channels. Cell.
Mol. Bioeng. 9, 73-84 (2016). [PDF]
51. Tien, J., Li, L., Ozsun,
O. & Ekinci, K.L., Dynamics of interstitial fluid
pressure in extracellular matrix hydrogels in microfluidic devices. J. Biomech. Eng. 137, 091009 (2015). [PDF]
50. Ozsun, O., Thompson, R.L., Ekinci,
K.L. & Tien, J., Non-invasive mapping of
interstitial fluid pressure in microscale tissues. Integr. Biol. 6, 979-987 (2014). [PDF]
49. Tien, J.,
Microfluidic approaches for engineering vasculature. Curr. Opin. Chem. Eng.
3, 36-41 (2014). [PDF]
48. Chan, K.L.S., Khankhel,
A.H., Thompson, R.L., Coisman, B.J., Wong, K.H.K., Truslow, J.G. & Tien, J.,
Crosslinking of collagen scaffolds promotes blood and lymphatic vascular
stability. J. Biomed. Mater. Res.
A 102,
3186-3195 (2014). [PDF]
47. Tien, J.
& Nelson, C.M., Microstructured extracellular matrices in tissue
engineering and development, an update. Ann. Biomed.
Eng.
42, 1413-1423 (2014). [PDF]
46. Wong, K.H.K., Truslow,
J.G., Khankhel, A.H. & Tien,
J. Biophysical mechanisms that govern the vascularization of microfluidic
scaffolds. In Vascularization: Regenerative Medicine and Tissue Engineering
(ed. Brey, E.M.), pp. 109-124 (CRC Press, Boca Raton,
FL, 2014). [PDF]
45. Truslow, J.G.
& Tien, J., Determination of vascular
permeability coefficients under slow lumenal filling.
Microvasc. Res. 90,
117-120 (2013). [PDF]
44. Wong, K.H.K., Truslow,
J.G., Khankhel, A.H., Chan, K.L.S. & Tien, J., Artificial lymphatic drainage systems for
vascularized microfluidic scaffolds. J. Biomed. Mater.
Res. A 101, 2181-2190 (2013). [PDF]
43.
Tien, J., Wong, K.H.K. & Truslow,
J.G. Vascularization of microfluidic hydrogels. In Microfluidic Cell Culture
Systems (eds. Bettinger, C.J., Borenstein, J.T. & Tao, S.L.), pp. 205-221 (Elsevier,
Oxford, U.K., 2013). [PDF]
42. Tien, J., Truslow, J.G. & Nelson, C.M., Modulation of invasive
phenotype by interstitial pressure-driven convection in aggregates of human
breast cancer cells. PLoS One 7, e45191
(2012). [Corrected PDF + Supporting
Information]
41. Leung, A.D., Wong, K.H.K. & Tien, J., Plasma expanders stabilize human microvessels in
microfluidic scaffolds. J. Biomed. Mater. Res. A 100, 1815–1822 (2012). [PDF]
40.
Wong, K.H.K., Chan, J.M., Kamm, R.D. & Tien, J., Microfluidic models of
vascular functions. Annu. Rev. Biomed. Eng. 14, 205–230 (2012).
[PDF]
39.
Truslow, J.G. & Tien,
J., Perfusion systems that minimize vascular volume fraction in engineered
tissues. Biomicrofluidics 5, 022201 (2011).
[PDF]
38.
Price, G.M. & Tien, J. Methods for forming human microvascular tubes in
vitro and measuring their macromolecular permeability. In Biological
Microarrays (Methods in Molecular Biology, vol. 671) (eds. Khademhosseini, A., Suh, K.-Y.
& Zourob, M.), pp. 281-293 (Humana Press, Totowa,
NJ, 2011). [PDF]
37.
Price, G.M., Wong, K.H.K., Truslow, J.G., Leung, A.D., Acharya,
C. & Tien, J., Effect of mechanical factors on
the function of engineered human blood microvessels in microfluidic collagen
gels. Biomaterials 31, 6182-6189 (2010). [PDF]
36.
Wong, K.H.K., Truslow,
J.G. & Tien, J., The role of cyclic
AMP in normalizing the function of engineered human blood microvessels in
microfluidic collagen gels.
Biomaterials 31,
4706-4714 (2010). [PDF] [Movie]
35. Truslow,
J.G., Price, G.M. & Tien, J., Computational
design of drainage systems for vascularized scaffolds. Biomaterials
30, 4435-4443 (2009).
[PDF]
34. Price, G.M. & Tien, J. Subtractive
methods for forming microfluidic gels of extracellular matrix proteins. In Microdevices in Biology and
Engineering (eds. Bhatia, S.N. & Nahmias,
Y.), pp. 235-248 (Artech House, Boston, MA, 2009). [PDF]
33. Price, G.M., Chu, K.K., Truslow, J.G., Tang-Schomer,
M.D., Golden, A.P., Mertz, J. & Tien, J., Bonding
of macromolecular hydrogels using perturbants. J. Am. Chem. Soc. 130, 6664-6665 (2008). [PDF + Supporting
Information] [Movies]
32. Price, G.M., Chrobak,
K.M. & Tien, J., Effect of cyclic AMP on barrier
function of human lymphatic microvascular tubes. Microvasc. Res. 76, 46-51 (2008). [PDF]
31. Golden, A.P. & Tien,
J., Fabrication of microfluidic hydrogels using molded gelatin as a sacrificial
element. Lab Chip 17, 720-725 (2007). [PDF]
30. Nelson, C.M. & Tien,
J., Microstructured extracellular matrices in tissue engineering and
development. Curr. Opin. Biotechnol. 17, 518-523 (2006).
[PDF]
29. Chrobak,
K.M., Potter, D.R. & Tien, J., Formation of
perfused, functional microvascular tubes in vitro. Microvasc. Res. 71, 185-196 (2006). [PDF] [Movies]
28. Tien, J.,
Golden, A.P. & Tang, M.D. Engineering of blood vessels. In Microvascular Research: Biology and
Pathology, Vol. 2 (eds. Shepro, D. & D'Amore, P.A.), pp. 1087-1093 (Elsevier Academic Press, San
Diego, CA, 2006). [PDF]
27. Tang, M.D., Golden, A.P. & Tien, J., Fabrication of collagen gels that contain patterned,
micrometer-scale cavities. Adv. Mater. 16, 1345-1348 (2004).
[PDF]
26. Gray, D.S., Tien,
J. & Chen, C.S., High conductivity elastomeric electronics. Adv. Mater. 16, 393-397 (2004). [PDF]
25. Chen, C.S., Tan, J.L. & Tien, J., Mechanotransduction at cell-matrix and cell-cell
contacts. Annu. Rev.
Biomed. Eng. 6, 275-302
(2004). [PDF]
24. Tang, M.D., Golden, A.P. & Tien, J., Molding of three-dimensional microstructures of
gels. J. Am. Chem. Soc.
125, 12988-12989 (2003). [PDF]
23. Gray, D.S., Tien,
J. & Chen, C.S., Repositioning of cells by mechanotaxis
on surfaces with micropatterned Young's modulus. J. Biomed. Mater. Res.
A 66,
605-614 (2003). [PDF]
22. Tan, J.L., Tien,
J., Pirone, D.M., Gray, D.S., Bhadriraju,
K. & Chen, C.S., Cells lying on a bed of microneedles:
an approach to isolate mechanical force. Proc. Natl. Acad. Sci. USA 100,
1484-1489 (2003). [PDF]
21. Clark, T.D., Ferigno,
R., Tien, J., Paul, K.E. & Whitesides,
G.M., Template-directed self-assembly of 10-μm-sized hexagonal plates. J. Am. Chem. Soc. 124, 5419-5426 (2002). [PDF]
20. Tien, J.,
Nelson, C.M. & Chen, C.S., Fabrication of aligned microstructures with a
single elastomeric stamp. Proc.
Natl. Acad. Sci. USA 99,
1758-1762 (2002). [PDF]
19. Tien, J.
& Chen, C.S., Patterning the cellular microenvironment. IEEE Eng. Med. Biol. 21, 95-98 (2002). [PDF]
18. Tan, J.L., Tien,
J. & Chen, C.S., Microcontact printing of
proteins on mixed self-assembled monolayers. Langmuir 18, 519-523 (2002). [PDF]
17. Tien, J.
& Chen, C.S. Microarrays of cells. In Methods of Tissue Engineering (eds. Atala, A. & Lanza, R.), pp.
113-120 (Academic Press, San Diego, CA, 2001).
16. Bowden, N., Tien,
J., Huck, W.T.S. & Whitesides, G.M.
Mesoscale self-assembly: the assembly of micron- and
millimeter-sized objects using capillary forces. In Supramolecular Organization and Materials Design (eds.
Jones, W. & Rao, C.N.R.), pp. 103-145 (Cambridge
University Press, New York, NY, 2001).
15. Clark, T.D., Tien,
J., Duffy, D.C., Paul, K.E. & Whitesides, G.M.,
Self-assembly of 10-μm-sized objects into ordered three-dimensional
arrays. J. Am. Chem.
Soc. 123, 7677-7682 (2001).
[PDF]
14. Gracias, D.H., Tien,
J., Breen, T.L., Hsu, C. & Whitesides, G.M.,
Forming electrical networks in three dimensions by self-assembly. Science 289, 1170-1172 (2000). [PDF]
13. Dike, L.E., Chen, C.S., Mrksich, M., Tien, J., Whitesides, G.M. & Ingber,
D.E., Geometric control of switching between growth, apoptosis, and
differentiation during angiogenesis using micropatterned substrates. In Vitro Cell. Dev. Biol. Anim. 35, 441-448 (1999). [PDF]
12. Deng, T., Tien,
J., Xu, B. & Whitesides,
G.M., Using patterns in microfiche as photomasks in
10-μm-scale microfabrication. Langmuir 15, 6575-6581 (1999). [PDF]
11. Breen, T.L., Tien,
J., Oliver, S.R.J., Hadzic, T. & Whitesides, G.M., Design and self-assembly of open,
regular, 3D mesostructures. Science 284, 948-951 (1999). [PDF]
10. Lahiri, J.,
Isaacs, L., Tien, J. & Whitesides,
G.M., A strategy for the generation of surfaces presenting ligands for studies
of binding based on an active ester as a common reactive intermediate. Anal. Chem. 71, 777-790 (1999). [PDF]
9. Tien, J.,
Breen, T.L. & Whitesides, G.M., Crystallization
of millimeter-scale objects with use of capillary forces. J. Am. Chem. Soc. 120, 12670-12671 (1998). [PDF]
8. Huck, W.T.S., Tien,
J. & Whitesides, G.M., Three-dimensional mesoscale self-assembly. J. Am. Chem. Soc. 120, 8267-8268 (1998). [PDF]
7. Marzolin, C., Terfort, A., Tien, J. & Whitesides, G.M., Patterning of a polysiloxane
precursor to silicate glasses by microcontact
printing. Thin Solid
Films 315, 9-12 (1998). [PDF]
6. Tien, J., Xia, Y. & Whitesides, G.M. Microcontact
printing of SAMs. In Self-Assembled Monolayers of Thiols (Thin Films, vol. 24) (ed. Ulman,
A.), pp. 227-254 (Academic Press, San Diego, CA, 1998).
5. Xia, Y., Venkateswaran,
N., Qin, D., Tien, J. & Whitesides,
G.M., Use of electroless silver as the substrate in microcontact printing of alkanethiols
and its application in microfabrication. Langmuir 14, 363-371 (1998). [PDF]
4. Mrksich, M.,
Dike, L.E., Tien, J., Ingber,
D.E. & Whitesides, G.M., Using microcontact printing to pattern the attachment of
mammalian cells to self-assembled monolayers of alkanethiolates
on transparent films of gold and silver. Exp. Cell Res. 235,
305-313 (1997). [PDF]
3. Tien, J., Terfort, A. & Whitesides,
G.M., Microfabrication through electrostatic
self-assembly. Langmuir
13, 5349-5355 (1997). [PDF]
2. Xia, Y., Tien,
J., Qin, D. & Whitesides, G.M., Non-photolithographic
methods for fabrication of elastomeric stamps for use in microcontact
printing. Langmuir 12, 4033-4038 (1996). [PDF]
1. Shaw, G.L. & Tien,
J., Energy levels of quark atoms. Phys. Rev. D 47,
5075-5078 (1993). [PDF]
FUNDING
Persufflation of Composite Tissue Transplants (DoD/Army W81XWH-17-1-0571)
(Re)vascularization
of Decellularized Scaffolds (NIH/NIBIB R03 EB024660)
Engineered
Invasive Human Breast Tumors with Integrated Capillaries and Lymphatics
(NIH/NCI U01 CA214292)
In Vivo Microsurgical
Anastomosis of Prevascularized Tissues (NIH/NIBIB R03
EB018851)
Development and Clinical
Validation of Algorithms for Non-Invasive Mapping of Vascular Permeability
(BU/BWH Partnership Program)
Non-Invasive Measurement
of Vascular Cell Adhesion to Biomaterials (BU Dean’s Catalyst Award)
Active Biomaterials (BU
Materials Science and Engineering Innovation Grant)
Effect of Interstitial Pressure on Epithelial Invasion from
Human Mammary Ducts (DoD/Army W81XWH-09-1-0565)
Engineering Functional Lymphatic Networks In
Vitro (NIH/NHLBI R21 HL092335)
Synthesis and Characterization of Patterned
Microvascular Networks (NIH/NIBIB R01 EB005792)
Self-Assembly of Mesostructured
Biomaterials (NIH/NIBIB R21 EB003157)
In Vitro Synthesis of a Microvascular Network
(NIH/NIBIB R21 EB002228)
Use of Microfabrication
and Self-Assembly in Tissue Engineering (Whitaker Foundation RG-02-0344)
Dynamic Substrates for Cell Culture (BU Special Program
for Research Initiation Grants)
Self-Assembly of Gels (BU Provost’s Innovation Fund)
Response of Endothelial Cells to Cell-Cell Contact
(NIH/NHLBI F32 HL010486)
LINKS
How to join our research program:
·
Postdoctoral
fellows:
Interested postdoctoral candidates
should send us a detailed cover letter, CV, and a list of three professional
references. We look for candidates with
a robust track record of publication and innovation.
·
Graduate
students:
Graduate students must
apply through one of the doctoral programs listed below—we are especially
interested in applicants with a strong quantitative background and excellent
technical skills:
Department of Biomedical Engineering
Program in Molecular Biology, Cell Biology, and Biochemistry
Division of Materials Science and Engineering
Late Entry Accelerated Program (LEAP) in
Biomedical Engineering
MD/PhD program at Boston University
School of Medicine
·
Undergraduate
students:
Undergraduate students
should send us a brief explanatory letter, transcript, and description of any
prior research experience. We seek
students who learn quickly, work hard, and have impeccable ethics.
Resources at BU:
Core facilities (lithography, imaging, and
materials characterization) in the Department of Biomedical Engineering
Core facilities (flow cytometry,
microarrays, transgenics, etc.) at the Medical Center
Core facilities
(lithography, SEM) in the Photonics Center
Collaborators:
Celeste Nelson, Department of
Chemical Engineering, Princeton University
Kamil Ekinci, Department of Mechanical Engineering, Boston
University
Databases and analytical software:
ISI Web of Knowledge (here,
for BU users)
The Lipid Library (with focus on
bioactive lipids)
Atlas of microsurgery
Statistical tests and when to use them
Don't know where to
publish? Ask JANE!
Journals of particular relevance to
microcirculation:
American Journal of
Physiology – Heart and Circulatory Physiology
Journal of Experimental Medicine
Lymphatic Research and Biology
Organizations:
National Institutes of Health (NIH)
National Institute of Biomedical Imaging and
Bioengineering (NIBIB)
National Heart, Lung, and Blood Institute
(NHLBI)
National Cancer Institute (NCI)
Information
on funded NIH grants: RePORTER database
Information
on deadlines,
study
sections, special
emphasis panels, funding strategies,
and opportunities
National Science Foundation (NSF)
Biomedical Engineering Society (BMES)
American Heart Association (AHA)
Lymphatic Education and Research Network
Organ Procurement and Transplantation
Network (OPTN)
NHS Blood and Transplant (NHSBT)
Upcoming events:
Seminars at BU in biomedical
engineering and systems
biology
Courses
at the Marine Biology Laboratory/Woods Hole
Courses at Cold Spring Harbor
Laboratory
49th Annual Northeast
Bioengineering Conference (Mar 30-Apr 1, 2023; Philadelphia, PA)
BMES 2023 Annual Meeting (Oct
11-14, 2023; Seattle, WA)
[Copyright
© 2023 by the Tien Group.]