Avian mtDNA Primers

The PDF file below provides details on a complete set of primers for amplifying and sequencing avian mitochondrial DNA. All of the primers have been designed to work with most or all birds and most perform very well in this regard.

The document is intended as a resource for those interested in using mtDNA for avian population genetics and systematics, whether that work involves sequencing complete mitochondrial genomes or single genes. A second goal is to encourage the use of genes other than cytochrome b, which is a poor choice for studies in which only one mitochondrial gene will be sequenced (see the file for my logic on cyt b).

Download PDF file

Happy sequencing!

Questions? Please email: msoren@bu.edu