Research >> Assay Development
smitra@bu.edu
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Sangha Mitra, Ph.D. Senior Research Associate, Dept of Molecular and Cell biology I developed a direct and
convenient spectrophotometric assay for methionine aminopeptidases. The
method employs the hydrolysis of a substrate that is a methionyl analogue of
p-nitroaniline , which releases the chromogenic product p-nitroaniline. This
chromogenic product can be monitored continuously using a UV–Vis
spectrophotometer set at 405 nm. The assay was compared
with a “standard” high-performance liquid chromatography (HPLC) discontinuous
assay and was used to determine the temperature dependence of the kinetic
constant as well as to screen two novel pseudopeptide inhibitors of MetAPs. Related Publications: 1.
Mitra, S.,
Dygas-Holz, A. M., Jiracek J., Zertova M., Zakova, L. and Holz, R.C. “A New
Colorimetric Assay for Methionyl Aminopeptidases: Examination of the Binding
of a New Class of Pseudopeptide Analog Inhibitors.”Analytical Biochemistry, 2006 Oct 1; 357(1):43-9. [Read abstract here] |
