Research and Development>> Assay Development
smitra@bu.edu
|
|
Sangha Mitra Biotechnology Professional and Consultant MBA candidate, December 2012 Health Sector Management Boston University School of Management I
developed a direct and convenient spectrophotometric assay for methionine
aminopeptidases. The method employs the hydrolysis of a substrate that is a
methionyl analogue of p-nitroaniline , which releases the chromogenic product
p-nitroaniline. This chromogenic product can be monitored continuously using
a UV–Vis spectrophotometer set at 405 nm. The assay
was compared with a “standard” high-performance liquid chromatography (HPLC)
discontinuous assay and was used to determine the temperature dependence of
the kinetic constant as well as to screen two novel pseudopeptide inhibitors
of MetAPs. Related Publications: 1. Mitra, S., Dygas-Holz, A. M., Jiracek J.,
Zertova M., Zakova, L. and Holz, R.C. “A New Colorimetric Assay for Methionyl
Aminopeptidases: Examination of the Binding of a New Class of Pseudopeptide
Analog Inhibitors.”Analytical
Biochemistry, 2006 Oct 1; 357(1):43-9. [Read abstract here] |
![MMj01855880000[1]](research_assaydev_files/image003.gif){kind=small}