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CURRICULUM VITAE

 

NAME:                         Jinghua YANG, Ph.D.

DATE OF BIRTH:       June 12, 1959

ADDRESS:                 150 S. Huntington Ave. 1A-320, Boston, MA 02130

TELEPHONE:                         (857)-364-6398

FAX:                            (857) 364-5627

Cell:                             (203) 214-8757

E-MAIL:                        jyang@bu.edu;  jinghua.yang@va.gov

 

EDUCATION and PROFESSIONAL EXPERIENCE:

    

     2006.11 – present, Associate Professor, Director

Surgical Oncology Laboratory

                                    Boston VA Medical Center

                                    Boston University School of Medicine

     2004.7 – 2006.10, Head of Surgical Oncology Research Laboratory.

                                    Department of Surgery

                                    Yale University, New Haven, CT 06520

     1998 – 2004           Assistant Professor

                                    Department of Surgery

                                    Yale University, New Haven, CT 06520

     1994 - 1998,           Post-doctoral fellow with Dr. Tom Maniatis

                                    Department of Molecular and Cellular Biology

                                    Harvard University, Cambridge, MA 02138

     1992 - 1994,           Post-doctoral fellow with Dr. Bernado Nadal-Ginard

                                    Howard Hughes Medical Institute, Dept of Cell. & Mole. Physiology,

                                    Harvard Medical School, Boston, MA 02115

     1988 - 1992,           Graduate Student (Ph.D.) with Dr. Robert. Cedergren

                                    Department of Biochemistry, University of Montreal, Montreal, CANADA

     1985 - 1988,           Assistant Lecturer & Lecturer in Biochemistry

                                    Department of Biochemistry, 4th Military Medical University, Xian, China

     1982 - 1985,           Graduate Student (M.Sc.) in Catalysis

                                    Beijing Research Institute of Chemical Engineering, Beijing

     1978 - 1982,           Undergraduate student (B.Sc.) in Chemistry

                                    Sichuan University, Chengdu, China

 

AWARDS and OTHER PROFESSIONAL ACTIVITIES:

 

Awards and honors:

1988 -        Fellowship, University of Montreal, Canada.

1992,         Outstanding NSERC Young Researcher Award.

                  National Science and Engineering Research Council of Canada (NSERC)

1992,         MRC Fellowship from Medical Research Council of Canada,

          Grant number: 9204FEN-1328-41318

1996,         Outstanding Young Scientist Award from National Science Foundation of China

1996-         Guest professor in Xian International BioMedical Institute, Xi’an, China.

1999,         Hellman Fellowship Award

 

Other professional activities:

Ad hoc Reviewer for the American Institute of Biological Sciences (AIBS, Scientific Peer Advisory and Review Services), 2007

Associate member of Yale Cancer Center, 2000-;

RNA society, 1996-

Guest professor in Xian International BioMedical Research Institute, Xian, 1996 - present:

Founder, Boston Biotechnology and Biomedicine Association, 1996-;

Vice president of Chinese-American Science and Technology Society, 2001-;

 

RESEARCH GRANT SUPPORTS DURING LAST 5 YEARS:

 

Currently active:

KO2 AI060701, Yang (PI),                          07/15/04 – 4/31/09          ($524,485, total)

NIH/NIAID

Title: Role of RNA editing by ADAR1 in infection

This support is to elucidate the role of the RNA editing enzyme ADAR1 in modulating cellular responses to infection.

Role: PI.

 

R01 GM 60426, Yang (PI),   01/01/00 – 12/31/05 extended to 10/31/07; ($1,207,707 total)  

         NIH/NIGMS,                                                                                

This support is to study the mechanism of A-to-I RNA editing enzyme: 1) to characterize the formation of I-mRNA in HeLa cells, and 2) to identify site-specific interactions in A-to-I RNA editing complex

 

BCTR0707230, Yang (PI),      07/01/2007 – 6/31/2010   $(298,000, direct)

Susan G. Komen for the Cure

Title: Activating innate immunity to fight breast cancer                                                                                

This support is to study a tumor specific complement activator (tsCA) that activates human complement system to kill cancer cells.

 

  Instrument Grant, Yang (PI),                                      07/01/2007      ($500,000 direct)

ShEEP, VA

This support is to purchase instruments for clinic proteomics including 1) Nano-LC QSTARŪ Elite Hybrid LC/MS/MS (nanoLC Q-TOF mass spectrometer), 2) Voyager-DE™ STR Biospectrometry Workstation (MALDI TOP mass spectrometer, and 3) multi-dimensional HPLC with spotting system.

 

Pending:

R21 CA124944-01, Yang (PI),                                                       

NIH/NIAID                                                                                       

This support is to identify serum markers for chemosensitivity of pancreatic cancer.

 

Concept Award on PTSD, Yang (PI),                                                       

DoD                                                                                    

This support is to the study on PTSD entitled “PTSD is triggered by traumatic stress-induced Rbrain RNA editing”.

 

Completed:

1. Hellman fellowship, Date: 1/1/00-

P.I.: Jinghua YANG; Title:  “The role of A-to-I RNA editase in inflammation.”

2. OHSE Fellowship; Date: 1/1/02 – 12/31/03.  

P.I.: Helen Su. Mentor: Jinghua Yang;

Title: Complement activator specific for tumor cells; 

3. OHSE Fellowship, 1/1/03 – 12/31/03

P.I.: Yingjun Su. Mentor: Jinghua YANG;

Title: Complement activator specific for tumor cells

4. OHSE Fellowship, 1/1/00 – 12/31/00

    P.I.: Xiaoxing Luo. Mentor: Jinghua YANG;

    Title: A-to-I RNA editase in inflammation.

5. NSFC # 39625023, 10/1/97 – 9/31/98

    P.I.: Jinghua YANG.

 

BIBLIOGRAPHY

Peer-reviewed original research:

*Yang J-H, Perreault JP, Labuda D, Usman N, Cedergren R. Mixed DNA/RNA polymers are cleaved by the hammerhead ribozyme. Biochemistry  29(51):11156-60 (1990).

            Annotation: This work was done as a graduate student in Dr Cedergen’s lab.

Xiang XB, Yang J-H, N.C. Chen and C.Z.Su, "Chemical Synthesis and Expression of HIV-env Gene in E.coli", Chinese J. Biological Chemistry  6(3):229 (1990).

Perreault JP, Labuda D, Usman N, Yang J-H, Cedergren R. Relationship between 2'-hydroxyls and magnesium binding in the hammerhead RNA domain: a model for ribozyme catalysis. Biochemistry 30(16): 4020-5 (1991).

            Annotation: This work was done as a graduate student in Dr Cedergen’s lab.

Yang J-H, Usman N, Chartrand P, Cedergren R. Minimum ribonucleotide requirement for catalysis by the RNA hammerhead domain. Biochemistry 31(21): 5005-9 (1992).

            Annotation: This work was done as a graduate student in Dr Cedergen’s lab.

Yang J-H and R. Cedergren, "Mechanism of RNA Catalysis", Doctoral Thesis, University of Montreal, 1992.

            Annotation: This work was done as a graduate student in Dr Cedergen’s lab.

Yang J.-H, Cedergren R, Nadal-Ginard B. Catalytic activity of an RNA domain derived from the U6-U4 RNA complex. Science 263(5143): 77-81 (1994).

            Annotation: This work was done as a postdoc under Dr. Bernado Nadal-Ginard.

Yang J.-H, Sklar P, Axel R, Maniatis T. Editing of glutamate receptor subunit B pre-mRNA in vitro by site-specific deamination of adenosine. Nature 374(6517): 77-81 (1995).

            Annotation: This work was done as a postdoc under Dr. Tom Maniatis and Richard Axel.

Yang J.-H, Sklar P, Axel R, Maniatis T. Purification and characterization of a human RNA adenosine deaminase for glutamate receptor B pre-mRNA editing. Proc.Natl. Acad. Sci. USA. 94(9): 4354-9 (1997).

            Annotation: This work was done as a postdoc under Dr. Tom Maniatis and Richard Axel.

F. Li, J-H Yang, X Liu, P He, S Ji, J Wang, J Han, N Chen, and L Yao.  Human Glioma Cell BT325 Expresses A Proteinase That Converts Human Plasminogen to Kringle 1-5-Containing Fragments.  Biochem. Biophy. Res. Comm. 278 (3): 821-825 (2000).

            Annotation: This work is generated from a collaborative study. I am the initiative and the designer of this project. It was performed by postdoc fellow Dr. Li under my guidance. I was heavily involved in data interpretation and manuscript preparation.

F. Li, J-H Yang, N Chen, and L Yao. One-step generation of human angiostatin directly from plasma.  Biochem. Biophy. 27(3):325-7 (2000).

            Annotation: This work is generated from the collaborative study as above. I am the initiative and the original designer and heavily involved in data interpretation and manuscript preparation.

Rabinovici R, Kabir K, Luo X, Chen M, Su Y, Zhang D, Yang J.-H*, ADAR1 is Involved in the Development of Microvascular Lung Injury. Circ Res  88: 1066 – 1071  (2001).

            Annotation: I am the co-corresponding author of this paper. I am the initiative and the original designer of this project, guided the research by providing daily trouble-shooting and data interpretation to postdoctoral fellows, and directly performed some of the experiments. I completed manuscript writing and submission, and rebuttal all by myself. 

K, K., Gelinas, J. P., Chen, M., Chen, D., Zhang, D., Luo, X., Yang J.-H., Carter, D. Rabinovici, R., Characterizing acute pulmonary inflammation in mouse exposed to endotoxin. Shock 17(4):300-303  (2003).

Rabinovici R, Zhang D, Su Y, Luo X, Zhao Q, Yang J-.H.: mob-1 and TNFa interact to induce microvascular lung injury. Shock 18(3):261-4  (2002).

Jing WU, De Xin Zhang, J.-H. YANG & Dai Ming FAN. Inhibitory efficacy of local angiostatin expression in treatment of human hepatic carcinoma cells. Chinese Journal of Digestive Diseases  3: 132–137  (2002).

J-H Yang*, Xiaoxing Luo, Yongzhan Nie, Yingjun Su, Qingchuan Zhao, Koroush Kabir, Dexin Zhang, Reuven Rabinovici.  Widespread Inosine-containing mRNA in Lymphocytes Regulated by ADAR1 in Response to Inflammation. Immunology  109:15-23  (2003).

            Annotation: As the first and corresponding author, I am the initiative and original designer of this project, and also performed some of the experiments. I completed manuscript writing and submission, and rebuttal by myself.  

Y. Nie, K. Wu, J-H. Yang, F. Tian, D. Fan.  Induction of T lymphocytes specific to gastric cancer using HLA-A matched allogeneic gastric tumor cells.  J. Immunotherapy 26(5) 403-411 (2003).

J. Wu, Y. Shi, K. Wu, D. Zhang, J-H Yang, D. Fan, Angiostatin up-regulation in gastric cancer cell SGC7901 inhibits tumorigenesis in nude mice. World J Gastroenterology 9(1): 59-64  (2003).

Jing-Hua Yang*, Yongzhan Nie, Qingchuan Zhao, Yingjun Su, Marc Pypaert, Haili Su and Reuven Rabinovici. Intracellular Localization of Differentially Regulated ADAR1 Isoforms in Inflammation.  J.Biol.Chem. 278(46):45833-42 (2003).

            Annotation: As the first and corresponding author, I performed some of the experiments and guided the completion of this project. The original thought development, data interpretation and manuscript writing, submission and rebuttal were done by myself.

Yongzhan Nie, Kaichun Wu and Jing-Hua Yang*. Specific cytolysis of tumor cells by xenogenically activated human serum. Clin. Cancer Res.

            Annotation: This paper is accepted but held by us due to a patent application process.  I am the corresponding author and independent PI of this paper.

Yongzhan Nie, Qingchuan Zhao, Yingjun Su and Jinghua Yang* Subcellular distribution of ADAR1 isoforms is synergistically determined by three nuclear discrimination signals and a regulatory motif. J.Biol.Chem. 279 (13):13249-55 (2004).

            Annotation: I am the corresponding author and independent PI of this paper.

Shuli Xia, Jing-Hua Yang*, Enbo Ma, Kevin White and Yingyi Xiao and Gabriel Haddad*.   Identification of the new targets of Drosophila pre-mRNA deaminase. Physiological Genomics 20;20(2):195-202 (2005).

            Annotation: I am the corresponding author of this paper. The work was done under my supervising in collaboration with Dr. Haddad.

Nie, Y. Ding, L. Kao, P. N. Braun, R. Jinghua, Yang*  ADAR1 interacts with NF90 through double-stranded RNA and regulates NF90-mediated gene expression independently of RNA editing.  Mol Cell Biol 25 (16) 6956-63 (2005).

            Annotation: I am the corresponding author and independent PI of this paper.

Yongzhan Nie, Graeme L. Hammond, & Jing-Hua Yang1* Double-stranded RNA editase ADAR1 increases susceptibility to viral infection.  J. Virology, 81(2):917-923 (2007).

            Annotation: I am the corresponding author and independent PI of this paper.

Patents:

1.      Nassim Usman, Robert Cedergren, J.P. Perreault, Jing-Hua Yang, Alexander Rich. University of Montreal.  Assignee: the Nuceozymes I.  US Patent 5,652,094 (7/29/1997).

2.      Nassim Usman, Alexander Rich, Robert Cedergren, J.P. Perreault, Jing-Hua Yang.   Assignee: Ribozyme Pharmaceuticals. Nuceozymes II; US Patent 6,140,49. (10/31/2000).

3.      Nassim Usman, Robert Cedergren, J.P. Perreault, Jing-Hua Yang, Alexander Rich.   Assignee: MIT.  Nuceozymes III. US Patent 7074601. (7/11/2006).

Book Chapter: 

in "Practical Protocols In Molecular Biology", Jing-Hua Yang, RNA Synthesis and Processing, (1996). Science Press & Science Press New Yok, Ltd., New York, U.S.A.

 

 

LECTURES and COURSES: 

 

Invited speech: “Role of ADAR1 in host response during viral infection”, Research Conference, Boston VA Medial Center, West Roxbury, MA.  Jan. 26, 2007.

Invited speaker and panelist: The 1st annual symposium of ACMS, Boston, MA.  Jan. 14, 2007.

Invited speech:  The 15th World Congress of Pharmacology, Xian Satellite Meeting, Xian, China, July 8-11, 2006

Invited speech: “RNA editing enzyme ADAR1 in host defense “Department of Biochemistry, Brandeis University, Boston, March 25-2005

Session Chair: Comparative and Functional Genomics Workshop, Hinxton, Cambridgeshire, U.K, November 2-5, 2003.

Chair and Organizer: Bio-Pharmaceutical Sciences, the Tri-state chapter of SCBA, New Haven, November 23, 2002

Invited speech: Beyond Identification and Transcription and Sequences, A-to-I RNA editing diversifies gene expression, 12nd BITS, DOE. Washington, D.C. October 25-28, 2002

Invited speech: “Strategies in Disease-related Proteomics”, Biomedicine and Biotechnology Association. Boston, June 20, 2001

Organizer and keynote speaker: “Discovery of New Drug Targets with Proteomic Mass Spectrometry”. Symposium on Proteomics. Xian, China, March 5, 2001.

Invited speech: “RNA editing and inflammation”. Boston University Medical Center. Boston University, Boston, MA, November  2001.

Invited speech: “Modifying the genetic information”, 90th Anniversary of TsingHua University, TsingHua University. Beijing, October 2000

Organizer and keynote speaker: “RNA editing and function”; 4th Symposium on Frontier of Life Science. NSF of China, Beijing, October, 1999

Invited speech: "Identification of A-I Editing in Mammalian Messenger RNAs",  Gordon Research Conferences, Ventura, CA, January 19-24, 1997

Invited speech: "Purification/Characterization of human RNA adenosine deaminase",    EMBO Workshop, Maastricht, The Netherlands, Sept. 5-9, 1996

Speaker: "In vitro editing of glutamate receptor B pre-mRNA by Deamination of Adenosine",  The first RNA Editing Workshop, Albany, NY, Oct.6-9, 1994