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Instrumentation
1. Nano-LC QSTAR® Elite Hybrid LC/MS/MS
System
The QSTAR® Elite System coupled with the Tempo nano
LC System provides rapid, robust protein identification. The
QSTAR Elite LC/MS/MS system is a Q-TOF mass spectrometer for proteomics and
metabolomics applications. The QSTAR Elite has improved sensitivity,
acquisition speed, resolution, dynamic range, and mass-measurement accuracy
for more-confident identifications. The system includes ProteinPilot software
can be used for iTRAQ and SILAC analyses. The instrument is equipped with the
Tempo LC system as a fully integrated LC/MS/MS solution for biomarker
discovery.
 2. Voyager-DE™ STR Biospectrometry Workstation
The MALDI-TOF mass spectrometer is an
Applied Biosystems VOYAGER-DE STR workstation. This is a matrix assisted laser
desorption ionization time-of-flight (MALDI-TOF) mass spectrometer with both
linear and reflectron modes providing excellent resolution, sensitivity, and
mass accuracy. Clients can choose from positive or negative ion detection
modes of operation as well as the ability to perform Post Source Decay (PSD)
experiments to obtain valuable peptide sequence information.
3. Agilent 1200
two-Dimensional Fluorescent HPLC
Agilent
1100/1200 Capillary/analytical with 2-Fluorescence detector for working with
cy3 and cy5. Whatever your priority,
speed, resolution or sensitivity, the new Agilent 1200 Series, with its
flexible, modular design, ensures configurations ideally suited to meet your
application requirements. Building on new ZORBAX column technology and novel
system design your analysis time will be shortened up to 20x. Resolution can
improve as much as 60%. More intuitive user interfaces will let you
instantaneously control and monitor the instrument status. New choices in
data analysis offer you rapid and efficient data review tools. The Agilent
diagnostic software enables Intelligent Services providing more uptime,
independent of the data system in use.
 4. 2-D Gel with Typhoon Imager
The Typhoon imager offers multi-color fluorescence,
chemifluorescence, chemiluminescence and filmless autoradiography in one
instrument. Laser excited, pixel-by-pixel fluorescence measurement eliminates
fluorescent blooming and provides better resolution than conventional
systems. The machine's dual lasers and two detectors enable the investigator
to employ multiple simultaneous colors and to choose from a wide range of
fluorescent probes
Scan modes:
Fluorescence, Chemiluminescence, Phosphorimaging
Scan resolutions:
200, 100, 50 microns/pixel (50, 100,
200 data points/cm)
Excitation lasers: 532
nm, 633 nm
Standard emission
filters:
555DF20, 580DF30, 610DF30, 670DF30,
526SP, 560LP
Beamsplitters: 560, 580
Platen size: 35 cm x
43 cm
5. Luminex 100IS multiplex liquid array system
The
Luminex™ platform combines the efficiencies of multiplexing up to 100 different Extracellular
or intracellular markers for simultaneous analysis, with similar
reproducibility to ELISA methodology. Protein Multiplex Immunoassays save
researchers sample, time and money. BioSource now offers over 100 kits for
use with the Luminex xMAP system, to allow for multiplexing of proteins
within a given species (human, mouse, rat, or monkey).
 
6. Protein Arrayer and
spotter
Programmable sampler for automated sample preparation, transfer
and spotting. This modified Gilson 223 Sample Changer is the perfect solution
for protocols such as serial dilutions, addition of samples, sampling into
vials, timed reactions and tube-to-tube transfers, spotting samples onto
MALDI targets and nitrocellulose membrane.
7. Flow Cytometry 
The FACScan has one laser at 488 nm and can collect 3
flourescent and 2 scatter properties simultaneously. It is important to
remember that only fluorochromes excited at ~488nm may be used on this
instrument.
The FACScan is currently housed at 150
S. Huntington Ave. 1A-116.
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