Clinic Proteomics Laboratory

 

Boston University Medical Campus            Boston VA Research Institute

670 Albany St. Pathology 404                        150 S. Huntington Ave. 1A

Boston, MA 02118                                                      Boston, MA 02130   
jyang@bu.edu                                                       Jinghua.yang@va.gov

Tel: (617) 414-7075                                                 Tel: (857) 364-5611

    Fax: (857)364-5627                                                  Fax: (857)364-5627

 

@Yanglab

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 Instrumentation

 

1. Nano-LC QSTAR® Elite Hybrid LC/MS/MS System

The QSTAR® Elite System coupled with the Tempo nano LC System provides rapid, robust protein identification. The QSTAR Elite LC/MS/MS system is a Q-TOF mass spectrometer for proteomics and metabolomics applications. The QSTAR Elite has improved sensitivity, acquisition speed, resolution, dynamic range, and mass-measurement accuracy for more-confident identifications. The system includes ProteinPilot software can be used for iTRAQ and SILAC analyses. The instrument is equipped with the Tempo LC system as a fully integrated LC/MS/MS solution for biomarker discovery.



 

 

 

 

 

 

 

2. Voyager-DE™ STR Biospectrometry Workstation

 The MALDI-TOF mass spectrometer is an Applied Biosystems VOYAGER-DE STR workstation. This is a matrix assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometer with both linear and reflectron modes providing excellent resolution, sensitivity, and mass accuracy. Clients can choose from positive or negative ion detection modes of operation as well as the ability to perform Post Source Decay (PSD) experiments to obtain valuable peptide sequence information.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

3. Agilent 1200 two-Dimensional Fluorescent HPLC

Agilent 1100/1200 Capillary/analytical with 2-Fluorescence detector for working with cy3 and cy5.  Whatever your priority, speed, resolution or sensitivity, the new Agilent 1200 Series, with its flexible, modular design, ensures configurations ideally suited to meet your application requirements. Building on new ZORBAX column technology and novel system design your analysis time will be shortened up to 20x. Resolution can improve as much as 60%. More intuitive user interfaces will let you instantaneously control and monitor the instrument status. New choices in data analysis offer you rapid and efficient data review tools. The Agilent diagnostic software enables Intelligent Services providing more uptime, independent of the data system in use.

 

 

 

 

 

 

 

 

4. 2-D Gel with Typhoon Imager

The Typhoon imager offers multi-color fluorescence, chemifluorescence, chemiluminescence and filmless autoradiography in one instrument. Laser excited, pixel-by-pixel fluorescence measurement eliminates fluorescent blooming and provides better resolution than conventional systems. The machine's dual lasers and two detectors enable the investigator to employ multiple simultaneous colors and to choose from a wide range of fluorescent probes

 

Scan modes: Fluorescence, Chemiluminescence, Phosphorimaging

Scan resolutions:

         200, 100, 50 microns/pixel (50, 100, 200 data points/cm)

Excitation lasers: 532 nm, 633 nm

Standard emission filters:

         555DF20, 580DF30, 610DF30, 670DF30, 526SP, 560LP

Beamsplitters:  560, 580

Platen size: 35 cm x 43 cm

 

 

 

 

5. Luminex 100IS multiplex liquid array system

The Luminex™ platform combines the efficiencies of multiplexing up to 100 different Extracellular or intracellular markers for simultaneous analysis, with similar reproducibility to ELISA methodology. Protein Multiplex Immunoassays save researchers sample, time and money. BioSource now offers over 100 kits for use with the Luminex xMAP system, to allow for multiplexing of proteins within a given species (human, mouse, rat, or monkey).

 

 

Image of 223 Sample Changer

 

6. Protein Arrayer and spotter

Programmable sampler for automated sample preparation, transfer and spotting. This modified Gilson 223 Sample Changer is the perfect solution for protocols such as serial dilutions, addition of samples, sampling into vials, timed reactions and tube-to-tube transfers, spotting samples onto MALDI targets and nitrocellulose membrane.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

7. Flow Cytometry 

The FACScan has one laser at 488 nm and can collect 3 flourescent and 2 scatter properties simultaneously. It is important to remember that only fluorochromes excited at ~488nm may be used on this instrument.


The FACScan is currently housed at 150 S. Huntington Ave. 1A-116.