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Clinic Application of Proteomics Boston University Medical Campus Boston VA Research Institute Boston, MA 02118 Boston, MA 02130 Tel: (617) 414-7075 Tel: (857) 364-5611
Fax: (857)364-5627
Fax: (857)364-5627 |
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Our laboratory is pleased to provide facilities to
investigators of |
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Application: Identification of autoimmune antigens from patients Description: Auto-immune diseases generate
immune responses against patient’s own proteins. Tumors
may also generate autoimmune responses. These immunogenic proteins are
potential diagnostic and therapeutic targets.
We will work with investigators to identify these antigens when the
tissue biopsies and blood samples from the same patient are available. Briefly, the disease tissue will be
resolved by two-dimensional gel and blotted onto membrane; the IgG from
patients and healthy donors will be isolated and labeled with cy5 and cy3,
respectively. Positive antigens will
be differentially detected by 2D Western, visualized by Typhoon Imager and
identified by mass spectrometry. The
FaAD platform can be applied to: - Tumors - Autoimmune diseases - Infectious diseases |
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Application: Differential profiling of blood/tissue
proteins from patients
DiGE Image |
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Application: Differential profiling of blood/tissue
proteins from patients Description: FaMD HPLC is a new approach developed in our
laboratory to differentially analyze whole protein samples from patients and
healthy donors on multi-dimensional HPLC.
In the similar way as DiGE, protein samples from patients and healthy
donors will be labeled with Cy3 and Cy5, combined and analyzed on a 2-D
Agilent HPLC equipped with two fluorescence detectors. With FaMD HPLC, we are able to fractionate
whole blood/tissue proteins from patients and healthy donors in the same run,
so that differentially expressed proteins can be quantitatively analyzed. The differentially expressed proteins will
be in the liquid form and ready for mass spectrometry analysis. |
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Application: Protein Identification Description: Our laboratory is equipped with 1) the
Tempo™ nano LC followed with QSTAR Elite LC/MS/MS System (Q-TOF), and
2) Applied Biosystems VOYAGER-DE STR workstation (MALDI-TOF). These We do the following analysis: - MALDI-TOF protein
identification for samples isolated from 1-D or 2-D gels. - Q-TOF protein
identification for samples isolated from 1-D or 2-D gels. - iTRAQ to identify
differentially expressed proteins for less complicate samples. - Date research and
analysis. |
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5. Luminex-based Multiplexed Marker
Validation (LMMV) Application: Validate a panel of
markers in patient samples
We are currently
planning to evaluate the specificity and sensitivity of a panel of colorectal
cancer related blood markers. |
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Our proteomics and
mass spectrometry infrastructure and expertise are used in collaborations
with colleagues at Boston University Medical Campus & Boston VA Medical
Center and elsewhere. |