To find out what these files are about, check: Inam ZS, Nelamangala SK, and Lin J-W. (2014) Application of a spike sorting procedure to analyze recordings in the crayfish ventral superficial flexor preparation: A high resolution approach to the study of neuromodulators on axons and synapses. The Journal of Undergraduate Neuroscience Education 12:140-149. How to use these files: There are two files in this folder. **Analysis Example: This file includes both suction electrode recordings of action potentials of the third nerve, and sharp electrode recordings of synaptic responses, in a muscle fiber, evoked by axons of the third nerve. The two sets of recordings were collected simultaneously and there are two hundred traces in each. The file also includes analysis code in the Procedure window (ctrl-m). Feel free to alter the code if you are comfortable with writing code. I do apologize for not commenting on the code. To run this program, you need to install IGOR. IGOR is a data analysis program popular among electrophysiologists. There is a very good educational discount available: You order a copy for your class and students are allowed to install it on their PCs/Macs. Furthermore, you don’t have to pay the license again until they publish a new version. (I believe you can try IGOR using a demo version, but you may not be able to save the results of your analysis.) **Analysis Manual: This PDF file provides step by step instructions showing you how to run the analysis in IGOR. It is understood that you may not be familiar with IGOR— neither were my students—but if you follow the instructions carefully, everything should work. This analysis is not polished in the sense that it is not especially automated, and is not menu driven. I make no apology for these "shortcomings" because students learn a great deal when they have to enter numbers themselves and perform each step of the analysis. ## If you would like to use the code to analyze your own data, you can just delete the traces in "Analysis Example" (Data==>Killwaves) and port your own data in (using data browser==>browse exp then drag traces is one way to do it). It may even be easier to copy the procedure and analysis script files into the IGOR experiment that contains your data. Your axon recording traces must be named: AP_?, and your muscle recording traces PSP_?, and ? should be consecutive numbers. AP and PSP traces must be recorded simultaneously.