CAS BI-311

GENERAL MICROBIOLOGY LAB SCHEDULE

                                                            (Spring, 2009)

 

Sections:  B1: MW 12-2 pm; B2: MW 3-5 pm

      B3: TR 9-11 am; B4: TR 2-4 pm

 

Laboratory Manual: H.J. Benson, Microbiological Applications, Complete Version

 

Materials needed: Lab coat, goggles & notebook, colored pencils. 

 

Grading Criteria

Lab Notebook                          5

Lab Exam 1                             25

Lab Exam 2                            25

Final Unknown Project            25

Quizzes                                   20

                        100 --------- Lab Grade 25% of total Course Grade

 

**Lab must be passed to pass the course

 

Lab Polices and Attendance

á       Attendance is absolutely mandatory, and will be taken before every laboratory period.  Each student may have ONE excused absence. Due to the nature of the lab, make ups may or may not be possible and will always be at the discretion of the TF. Any conflicts with the dates below, especially regarding lab exams should be brought to the TFŐs attention during the first week of the lab.  Unexcused absences or poor participation may result in a deduction of the studentŐs grade at the instructorŐs discretion. 

 

Dress

á       Gloves must be worn at all times and hands washed before and after lab exercises. 

á       Lab coats are optional, but strongly suggested. Cheap paper lab coats are available at the bookstore.   

á       No open toed shoes, or shorts.

á       Long hair must be tied back. 

 

Other

á       No food or drinks will be tolerated.  This includes gum, coffee, and water bottles.

á       Workspaces must be cleaned thoroughly with disinfectant (70% EtOH) before and after each lab session. 

á       Any injuries no matter how minor should be reported to the TF immediately and any pre-existing health conditions should be reported to the TF where pertinent. 

á       Lab notebooks are required. Colored pencils are strongly recommended. 

á       Some labs may require coming in outside of scheduled lab time. 

  • The topics to be covered in each lab section MUST be read in the BensonŐs Lab Manual prior to attending your section.

 

 

 

 

 

 

 

       LAB

 

   TIME     TABLE

1

1/21

Week 1

a.)   Introduction: Overview of the syllabus. Lab safety. Structure and organization. Introduction to basic laboratory techniques.  

b.)   Ubiquity of Bacteria Ex. 7

c.)   Synthetic Epidemic Ex. 78

d.)   Overview of the Microscope Ex. 1, 3 & 5

 

2

1/26

Week 2

a.)   Ubiquity of Bacteria Ex. 7 (Results)

b.)   Aseptic technique Ex. 9

c.)   Pure Culture Techniques Ex. 10

d.)   Fungi Ex. 8

e.)   Microscopy II Ex. 1 (cont) Look at prepared slides.

 

3

 

 

1/28

Week 2

 

a.)   Pure Culture Techniques Ex. 10 (cont)

b.)   Smear Preparation Ex. 11

c.)   Gram Staining Ex. 15

d.)   Fungi (cont) Ex. 8

 

 

4

 

2/2

 

Week 3

a.)   Announced Quiz:  Aseptic technique

b.)   Pure Culture Techniques Ex. 10 (cont)

c.)   Acid-Fast Staining Ex.17

d.)   Spore Staining Ex.16

 

5

2/4

Week 3

a.)   Pure Culture Techniques Ex. 10 (Results)

b.)   Capsular Staining Ex. 14

c.)   Negative Staining Ex. 13

 

6

2/9

Week 4

a.)   Bacterial Testing of Water Ex. 61   

b.)   Culture Media Preparation Ex. 19 (Demo)

c.)   Motility Test Ex. 18

 

7

2/11

Week 4

a.) Bacterial Testing of Water Ex. 61: (cont)

b.) Motility Test Ex. 18 (Results)

b.) Oxygen Requirements Ex. 21

 

 

2/16

Week 5

No Lab - Presidents Day

8

2/18

Week 5

a.)   Oxygen Requirements (Results) Ex. 21

b.)   Bacterial Testing of Water Ex. 61: (cont)

c.)   Observation of motile protests, algae and cyanobacteria Ex 6

 

9

2/23

Week 6

a.) Temperature: Effects on Growth Ex. 29

b.) Temperature: Lethal Effects Ex. 30

c.) Bacterial Testing of Water: Ex. 61 (cont)

10

2/25

Week 6

a.) Temperature Labs (Results)

b.) Effects of UV irradiation Ex. 33

c.) Nitrogen Fixation Part I: Ex. 50

 

11

3/2

Week 7

a.)   Lab Exam #1

b.)   Effects of UV irradiation Ex. 33 (Results)

c.)   Effects of pH on Growth: Ex 31

d.)   Effects of Osmotic Pressure on Growth: Ex 32

12

3/4

Week 7

a.)   Nitrogen & Ammonification labs Part II: Ex 50 & 52

b.)   pH Ex. 31 (Results)

c.)   Osmotic pressure Ex. 32 (Results)

 

 

3/9

3/11

Week 8

No Labs Spring Break

14

3/16

Week 9

a.)   Introduction to Morphological Study of Unknown Ex. 39

b.)   Introduction to BergeyŐs Manuel Ex. 44

c.)   Unknown Lab Computer Module

d.)   Nitrogen Fixation Part III: Lab 52 (cont)

 

15

3/18

Week 9

a.)   Determination of the Unknowns: Begin Preliminary Staining: Ex 39

b.)   Hand Scrubbing: Skin Microflora Ex.38

 

 

16

 

3/23

 

Week  10

a.)   Morphological Study Of Unknowns I: Ex 39 & 41

b.)   Hand Scrubbing: Skin Microflora Ex.38 (Results)

 

18

3/25

Week 10

a.)   Morphological Study of Unknowns II: Ex 39, 40 & 41 (cont)

19

3/30

Week 11

a.)   Morphological Study of Unknowns III: Ex. 42 & 43

b.)   Multitest System: Enterotube II: Ex. 46

 

20

4/1

Week 11

a.)   Work on unknown

b.)   Antiseptics & Disinfectants: Ex. 35 & 37

 

21

4/6

Week 12

 

a.)   Microbial Testing of Food: Ex. 60

b.)   Microbiology of Milk: Ex 63

c.)   Alcohol Fermentation: Ex 65 (Demo)

22

4/8

Week 12

a.)   Microbial Testing of Food (results)

b.)   Microbiology of Milk (results)

c.)   Work on unknown (if needed)

 

23

4/13

 

Week 13

a.)   Simulated Blood Typing: Ex. 76 & 77

b.)   Evaluation of Antibiotics: Ex 36

 

24

4/15

Week 13

a.)   Unknown Project Report Due

b.)   Evaluation of antibiotics (results)

c.)   Review

 

25

4/20

4/22

Week 14

 

No classes

26

 

 

 

4/27

Week 15

 

 

a.)   Final Lab Exam

 

 

Lab contributes 25% of te course grade. It needs to be passed in order to pass the course