REAGENTS

Proteins That Can Interact with Rel/NF-kB/IkB Signaling Proteins

PROTEIN INTERACTING PARTNER(S) REFERENCE

Heat shock protein

v-Rel; RelA; IKK

Lim et al, 1990 ; Guzhova et al, 1997; Hinz et al, 2007

Unknown 200 kDa

v-Rel

Morrison et al, 1989

Sp1

v-Rel, c-Rel, RelA

Sif & Gilmore, 1994; Perkins et al, 1993;   Majello et al 1994

TBP

v-Rel, c-Rel, RelA, Dorsal

Xu et al, 1993; Kerr et al, 1993; Norris & Manley, 1992; Schmitz et al, 1995

TFIIB

v-Rel, RelA 

Xu et al, 1993; Schmitz et al, 1995 : Xia et al, 2004

p300                                                                        

RelA, c-Rel    

Perkins et al, 1997; Yu et al, 2004

AO7                                                                      

RelA       

Asamitsu et al, 2003

HDACs                                                                 

RelA, p50,  IkBa

Ashburner et al, 2001; Chen et al, 2001; Viatour et al, 2003

TAFII105                                                                    

RelA  

Yamit-Hezi et al, 2000

Centrosomal P4.1-associated protein                            

RelA  

Koyanagi et al, 2005

Trip6                                                                       

v-Rel, RelA     

Koedood Zhao et al, 1999; Kassel et al, 2004

NFATc                                                                     

c-Rel    

Pham et al, 2005

Bcl-2                            

p50                                                     

Hour et al, 2000

CREB                            

p50                                                     

He & Weber, 2004

DLC-1                             

IkBa                                                     

Crepieux et al, 1997

ESE-1                          

p50                                                     

Rudders et al, 2001

HMGI(Y)                                

p50,RelA

Thanos & Maniatis, 1992; Du et al, 1993

Importin alpha isoforms                   

p50,RelA,p52, c-Rel, RelB

Fagerlund et al, 2005; 2008

Nucleophosmin                               

p50,RelA

Dhar et al, 2004

HMGA2                                                                     

p50      

Noro et al, 2003

HMGB1                                                              

p50              

Agresti et al, 2003

Menin                                                              

p50, p52, RelA            

Heppner et al, 2001

Steroid Receptor Coactivator-1 (SRC-1)                                                              

p50              

Na et al, 1998

TLS                                                              

RelA              

Uranishi et al, 2001

EPRAP                                                             

p105             

Minami et al, 2008

Gas6                                                              

p105             

Demarchi et al, 2001

G protein-coupled receptor kinase 5                                                            

p105             

Parameswaran et al, 2006

IKKepsilon                              

p52          

Wietek et al, 2006

Retinoid X Receptor                                               

IkBbeta, p50, RelA, Bcl-3     

Na et al, 1998 , 1999

ASPP          

RelA 

Sullivan & Lu, 2007

Daxx         

RelA 

Park et al, 2007

Dynein motor complex       

RelA 

Mikenberg et al, 2007

FAD24

RelA 

Johmura et al, 2008

p28(GANK)    

RelA 

Chen et al, 2007

LZAP         

RelA 

Wang et al, 2007

MYBBP1a           

RelA 

Owen et al, 2006

PDLIM2         

RelA 

Tanaka et al, 2007

SIMPL             

RelA 

Kwon et al, 2004

TTF-1               

RelA 

Islam & Mendelson, 2002

CHFR  

RelA 

Kashima et al, 2009

Glucocorticoid Receptor               

RelA 

Ray & Prefontaine, 1994; Sheinman et al, 1995

Androgen Receptor                                           

RelA, c-Rel          

Palvimo et al, 1996; Mukhopadhyay et al, 2006

Progesterone Receptor                                           

RelA             

Kalkhoven et al, 1996

Estrogen Receptor                                           

c-Rel, RelA            

Galien & Garcia, 1997; Kalaitzidis & Gilmore, 2005; Feldman et al, 2007; Quaedackers et al, 2007

AH Receptor                                                    

RelA, p50, RelB   

Tian et al, 1999; Vogel et al, 2007

ASC-1                                                    

RelA, p50   

Jung et al, 2001

CDX2                                        

RelA, p50   

Mutoh et al, 2007

ETS, PU.1, ELF-1                                            

RelA, p50   

Bassuk et al, 1997

GILZ                                                                   

RelA, p50 

Riccardi et al, 2001; Marco et al, 2006

LSF (SEF)                                                                 

RelA, p50 

Bing et al, 2000

Notch1                                                                 

RelA, p50, IKKa

Wang et al, 2001; Guan et al, 1996; Song et al, 2008

NFBP                                                                           

RelA, p50  

Sweet et al, 2003

PARP-1                                                                          

RelA, p50  

Hassa et al, 2001; Kitamura et al, 2005

SMRT                                                                         

RelA, p50  

Lee et al, 2000

UXT                                                                   

RelA, p50, c-Rel  

Sun et al, 2007

LYL1                                                                           

p105   

Ferrier et al, 1999

ZUD                                                                          

p105, RelA   

Zhang et al, 2004

p53                                                                           

IkBa, RelA 

Chang, 2002 ; Zhou et al, 2003; Jeong et al, 2004; Dreyfus et al, 2005

p73                                                                        

IKKa

Furuya et al, 2007

Huntingtin                                                                         

IkBa, RelA 

Goswami et al, 2006

HOXB7                                                                   

IkBa    

Chariot et al, 1999

Beta TrCP                                                                 

IkBa   

Kroll et al, 1999

Beta Arrestin2                                                                

IkBa, p105  

Gao et al, 2004; Parmameswaran et al, 2006

RASGAPSH3BP2                                                      

IkBa   

Prigent et al, 2000

ANT                                                                          

IkBa   

Bottero et al, 2001

CDK4

IkBa   

Li et al, 2003

Calpain 3

IkBa   

Marcilhac et al, 2006

Dynein light chain LC8

IkBa   

Jung et al, 2008

Fibronectin

phospho-IkBa   

Olson et al, 2008

HnRNPA1                                                                     

IkBa     

Hay et al, 2000

c-Abl                                                                               

IkBa 

Kawai et al, 2002

MIG-6                                                                     

IkBa       

Tsunoda et al, 2002; Mabuchi et al, 2005

HDACs, nuclear co-repressors                                                                   

IkBa       

Aguilera et al, 2004

HDAC3                                            

IkBa   

Gao et al, 2005

Nrpo (Swine fever virus)                                      

IkBa   

Doceul et al, 2008

Bcl10                                                 

Bcl-3; IKKb   

Yeh et al, 2005; Wegener et al, 2006

LCK                                                   

Bcl-3      

Zhao et al, 2005

Cot/Tpl2                                                                            

c-Rel, p50/p105, p52/p100, RelA

Bouwmeester et al, 2004

ABIN2                                                                   

c-Rel, RelA, p105    

Bouwmeester et al, 2004; Lang et al, 2004

PAPOLA                                                                   

c-Rel       

Bouwmeester et al, 2004

IQGAP2                                                                  

IkBb, RelA  

Bouwmeester et al, 2004

CDK9                                                                

p50, RelA    

Amini et al, 2002

PKA                                                                

IkBa, IkBbeta , p50

Zhong et al, 1997; Guan et al, 2005

Proteasome                                                             

p100delta C-term

Qing et al, 2007

Erbin                                                              

c-Rel

Ress & Moelling, 2006

JNK1                                                               

c-Rel

Meyer et al, 1996

NIK                                                             

c-Rel

Sanchez-Valdepenas et al, 2006

PKA-Cbeta

c-Rel

Yu et al, 2004

S9                                                                                

p100

Fong et al, 2002

Actin                                                          

RelA    

Fazal et al, 2007

Alpha-actinin-4                                                         

RelA    

Babakov et al, 2008

RAI                                                                         

RelA    

Yang et al, 1999

NRF                                                                   

RelA  

Nourbakhsh & Hauser, 1999

nAKAP95                                                                   

RelA  

Bouwmeester et al, 2004

53BP2                                                                         

RelA

Yang et al, 1999

PPAR-a, b, d                                                                      

RelA 

Delerive et al, 1999; Planavila et al, 2005

MOZ                                                                    

RelA 

Chan et al, 2007

AES                                                                           

RelA 

Tetsuka et al, 2000

COMMD1

RelA

Burstein et al, 2005; de Bie et al, 2006

DEK

RelA

Sammons et al, 2006

gankyrin

RelA

Higashitsuji et al, 2007

PML                                                                   

RelA

Wu et al, 2003

Egr-1                                                                    

RelA

Lee et al, 2005

Egr -3, Egr-4                                                                 

RelA, p50

Wieland et al, 2005

FBI-1                                                                    

RelA

Chapman & Perkins, 2000

OCT1                                                                    

RelA

van Heel et al, 2002; dela Paz et al, 2007

X11L                                                                           

RelA

Tomita et al, 2000

HEXIM1                                                                           

RelA

Ouchida et al, 2003

ING4                                                                     

RelA  

Garkavtsev et al, 2004

Casein kinase II                                                                      

RelA  

Wang et al, 2000

c-Src                                                                    

RelA  

Bijli et al, 2006

p38-MAPK                                                           

RelA  

Rajaiya et al, 2007; von Brandenstein et al, 2008

PIAS1                                                                          

RelA  

Liu et al, 2005

PKC-zeta                                                                          

RelA  

Leitges et al, 2001

Ribosomal protein S3                                                                      

RelA 

Wan et al, 2007

RIP140                                                                      

RelA 

Zschiedrich et al, 2008

RNA Helicase A                                                                      

RelA 

Tetsuka et al, 2004

TAD binding proteins                                                        

RelA 

Owen et al, 2005

T-box transcription factor                                                      

RelA 

Hwang et al, 2005

VP1686 (Vibrio)                                                    

RelA 

Bhattacharjee et al, 2006

PKR                                                                          

IKK  

Bonnet et al, 2000 ; 2006

Protein Phosphatase 2Cb                          

IKK complex 

Prajapati et al, 2003

Protein Phosphatase X                         

c-Rel

Hu et al, 1998

Protein Phosphatase 2A                         

RelA; IKK, NF-kB; NEMO

Yang et al, 2001; Li et al, 2006; Hong et al, 2007

E2F1                                                                           

p50, RelA

Kundu et al, 1997; Lim et al, 2007

Myc                                                                          

RelA

Chapman et al, 2002

SF-1                                                                          

NF-kB

Hong et al, 2003

p21 (recombinant)                                                                      

NF-kB

Khanna et al, 2005

P47phox                                                                     

RelA

Gu et al, 2003

BRCA1                                                               

RelA      

Benezra et al, 2003

Telomerase                                                               

RelA

Akiyama et al, 2003

Replication Factor C                                                               

RelA

Anderson & Perkins, 2003

Beta-catenin                                                             

RelA

Deng et al, 2002

14-3-3 proteins                                                         

RelA, IkBa

Aguilera et al, 2006; Matta et al, 2008

Karyopherin alpha                                                            

RelA

Cunningham et al, 2003

SIRT1                                                            

RelA

Yeung et al, 2004

Sir2                                                           

RelA

Yang et al, 2004

REPS2                                                        

RelA

Penninkhof et al, 2004

ELKS                                                             

IKKa, IKKb, IKKg

Ducut Sigala et al, 2004

ENaC                                                                  

IKKb

Lebowitz et al, 2004

NIBP                                                               

IKKb, NIK

Hu et al, 2005

HCV Core protein                                                                

IKKb

Joo et al, 2005

ABIN-1                                                       

NEMO

Mauro et al, 2006

Bcl-10                                                                 

NEMO

Wu & Ashwell, 2008

CARMA1 and 3                                                                  

NEMO

Stilo et al, 2004

CIKS                                                                  

NEMO

Mauro et al, 2003

HIF2alpha                                                                  

NEMO

Bracken et al, 2005

PIDD                                                                

NEMO (and RIP-1)

Janssens et al, 2005

RIP-1                                                             

NEMO

Wu et al, 2006

c-Src                                                                

NEMO

Funakoshi-Tago et al, 2005

TANK                                                                    

NEMO

Chariot et al, 2002

TRAF1,2                                                                 

NEMO

Devin et al, 2001

Lys-63 ubiquitin                                                                

NEMO

Wu et al, 2006

ZNF216                                                                 

NEMO

Huang et al, 2004

TFG                                                              

NEMO, TANK

Miranda et al, 2006

vIRF3 (KSHV) IKKbeta Seo et al, 2004
AKT IKKbeta; RelA Vandermoere et al, 2006; Lam et al, 2007; Kwon et al, 2007
Fas-Associated Factor-1 IKKbeta Park et al, 2007
alpha-catulin IKKbeta Wiesner et al, 2007
MUC1 IKKbeta; NEMO Ahmad et al, 2007
mTOR IKK complex A Baldwin, personal communication
cdc37 IKK complex Hinz et al, 2007
CUEDC2 IKKa, IKKb Li et al, 2008
Heat Shock Protein 27 IKKa, IKKb Park et al, 2003; Kammanadiminti & Chadee, 2006
Hematopoietic kinase 1 IKK complex Brenner et al, 2005
Tgat (Rho guanine exchange factor) IKK complex Yamada et al, 2007
STAP-2 IKKa/b Sekine et al, 2006
TNAP NIK, Traf2, Traf3 Hu et al, 2004

c-Jun                                               

p50, RelA, IkBa, IkBb, NEMO

Stein et al, 1993; Kaszubuska et al, 1993 ; Cooper et al, 2005; Shifera et al, 2007

JunD                                                                 

p50-RelA    

Toualbi-Abed et al, 2008

c-Fos                                                                       

RelA,NEMO    

Stein et al, 1993; Shifera et al, 2007

C/EBPalpha                                      

p50 

Paz-Priel et al, 2005

C/EBPbeta                                        

p50, RelA,c-Rel

Stein et al, 1993; LeClair et al, 1992 ; Zwergal et al, 2006; Cha-Molstad et al, 2007

GABPbeta                                                                 

RelA

Bruder et al, 1993

ATFa, ATF2, ATF3                                    

p50, RelA  

Kaszubuska et al, 1993

YY1                                                                             

RelB 

Sepulveda et al, 2004

Elf-1                                                                             

p50 

John et al, 1995

HBXAP                                                                     

p50, RelA

Huang et al, 2004

Nucleophosmin/B23                                                       

p50, RelA

Lin et al, 2006

KPNA3                                                                        

p50/p105, RelA

Bouwmeester et al, 2004

IRF-1                                                                           

p50, RelA

Drew et al, 1995; Sgarbanti et al, 2008

IRF-2                                                                            

p50

Drew et al, 1995

IRF-8                                                                          

c-Rel

Liu & Ma, 2006

CDX2                                                                           

RelA

Kim et al, 2004

Stat1 (aceytlated)                                                        

RelA      

Kramer et al, 2006

Stat3                                                         

RelA      

Yu et al, 2002 ; Yu & Kone, 2004; Yang et al, 2007

Stat6                                                         

RelA, p50      

Shen & Stavnezer, 1998

SRF                                                                       

RelA   

Franzoso et al, 1996

Twist                                                                  

Dorsal   

Jiang & Levine, 1993

Twist 1,2                                                                  

RelA 

Sosic et al, 2003

Pin1                                                                 

RelA, c-Rel 

Fan et al, 2009

Calmodulin                                                            

c-Rel 

Antonsson et al, 2003; Khan et al, 2005

Cdk2/cyclinE                                                            

c-Rel 

Chen & Li, 1998

Myotrophin/V-1                                                           

c-Rel, RelA, p50

Knuefermann et al, 2002

Snail                          

Dorsal

 

DSP-1                                                              

Dorsal, p50

Lehming et al, 1994

Tax                                                                                 

p50

Suzuki et al, 1993

                                                                                   

RelA 

Suzuki et al, 1994

                                                                                 

c-Rel 

Suzuki et al, 1994

                                                                 

IkBa

Hirai et al, 1994; Petropoulos & Hiscott, 1998                                        

                                                   

p100 

Béraud et al, 1994; Lanoix et al, 1994; Murakami et al, 1995; Xiao et al, 2001

                                                                                                    

p105 

Hirai et al, 1992

                                                                                                    

IKK-Tak1

Wu & Sun, 2007; Chu et al, 1998

                                                                                                    

NEMO

Chu et al, 1999; Harhaj & Sun, 1999; Xiao et al, 2000

BZLF1  (EBV)                                                               

RelA      

Gutsch et al, 1994

HIV-1 Resistance Factor                                                                      

p50 

Lesner et al, 2005  

HHV-8 vFLIP                                                                      

NEMO 

Liu et al, 2002; Field et al, 2003  

HHV-8 K13 protein                                                                    

IKK complex 

Matta et al, 2007  

Adenovirus E3-14.7K                                                 

NEMO, p50    

Li et al, 1999 ; Carmondy et al, 2006

HBV  X protein                                                                

IkBa

Weil et al, 1999

HCV NS5B                                                                

IKKa

Choi et al, 2006

Torque Teno Virus ORF2                                                                

IKKa, IKKb

Zheng et al, 2007

M protein (SARS-Coronavirus)                                                           

IKKb

Fang et al, 2007

Microplitis demolitor bracovirus H4, N5                                                                 

Dif, Relish

Thoetkiattikul et al, 2005

Rual et al (2005) have used a high-throughput yeast two-hybrid screen to identify many interacting partners for the following NF-kB signaling proteins: 

 IKKgamma(NEMO):  NEMO, PPM1B, TANK, RIPK, MAP3K14, TNFRSF1A, HSPCA, COPS3, HSPA1A, ZA2OD2, TRPC4AP, C6ORF4, TAB3, UBB, RNF11

RelA:  STAT3, NR3C1 (glucocorticoid receptor), HIS1, PML, IkBbeta, HDAC3, HDAC1, beta-TRCP, POU2F1, PRKCzeta, PIAS3, IRF2, ITGB3BP, RNF25, KPNA2, ETHE1, PPP4C, FOS, TLE1, CAMkinase4, ING4, MEN1, TRIP4, PPARA

IkBalpha:  G3BP2, S6 kinase, p53, BTRC, K-alpha-1, RTN4, TUBA2, CAPN1, FBXW11, ARRB1, RAB6IP2, ARRB1, RAB6IP2, POM121

IkBbeta:  BTRC, RXRA, RelA

IkBepsilon:  IL1A, JUP

Feldman et al, 2007 used co-precipitation/mass spec analysis to identify several proteins on the NF-kB DNA complex (although in many cases a direct interaction was not verified).  These proteins include the following:  ADP-ribosyltransferase (NAD+; poly(ADP-ribose) polymerase); U5 snRNP-specific protein, 116 kDa; Similar to heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A); Splicing factor 3B subunit 3 (spliceosome-associated protein 130) (SF3b130) (pre-mRNA splicing factor SF3b 130 kDa subunit); RNA helicase A; Unnamed protein product; Splicing factor proline/glutamine rich (polypyrimidine tract-binding protein associated) PTB-associated splicing factor; Actinin, alpha 4; Human DNA topoisomerase I (70 kDa) in noncovalent complex with a 22-base pair DNA duplex; KIAA0432; Thyroid autoantigen 70 kDa (Ku antigen); thyroid autoantigen 70 kDa (Ku antigen); ATP-dependent DNA helicase II; PARP-1; Similar to nucleolin (protein C23); Hsp89-alpha-delta-N; Chaperone protein HSP90 beta; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 17 isoform 1; Structure-specific recognition protein 1; Interferon-inducible IFI 16; Similar to splicing factor proline/glutamine rich; Ras-GAP SH3 binding protein; Unknown (protein for IMAGE:3503708); Coronin, actin binding protein, 1C; coronin, actin-binding protein, 1C; Proliferation-associated 2G4; Neural precursor cell expressed; Beta actin; B23 nucleophosmin; Replication factor C; Ribosomal protein, large, P0