Techniques and Images
We use several methods for imaging activity in the intact, or mostly intact, brain.
In the first, we load olfactory receptor neurons in living mice with calcium-sensitive dyes, which travel
to the axon terminals in the olfactory bulb. In the second, we use a genetically-encoded probe of transmitter
release (synaptopHluorin) that is expressed in all olfactory receptor neurons. Each method allows us to image
patterns of olfactory input to different glomeruli of the olfactory bulb in vivo, and also to investigate
how this input is regulated by synaptic processing in vitro. For more specific info on particular
projects, see Research.
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| Olfactory receptor axons loaded with Calcium Green dextran. |
Olfactory bulb of OMP-synaptopHluorin mouse. |
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Calcium Imaging: (click on image to see larger view)
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Imaging odor-evoked calcium signals in vivo. |
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| Two-photon imaging of calcium signals within one glomerulus in vivo. |
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Temporal dynamics of odor representations. |
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SynaptopHluorin Imaging: |
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Imaging odor representations using synaptopHluorin. |
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| Bilateral odor representations imaged with synaptopHluorin. |
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| Mapping responses to a homologous odor series. |
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Mitral Cell Imaging:
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| Retrograde Mitral Cell Labeling. |
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| Mitral cell apical dendrites innervating a glomerulus. |
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