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This project explored how protein structures evolve from one fold to
another. By swapping two consecutive residues in the dimeric DNA-binding
protein arc-repressor of phage p22, we converted an intermonomer beta
strand to two interacting helices. The demonstration that simple mutations can
interconvert protein secondary structures has important implications for how
proteins evolve into new folds, in vivo. This project was a collaboration with Drs.
Matthew Cordes, now at the University of Arizona, and
Robert Sauer
at MIT.
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